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  • great britain turmeric extract drying powder good ...

    Extracts & Flavorings Manufacturer . 2021-9-17u2002·u2002Synergy has flavored the world's finest foods, beverages and nutritional products for more than 130 years with a diverse library of value-added flavors, extracts and essences that are tailored to meet a range of sweet, savory and dairy applications.

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  • Unbiased analysis by high throughput sequencing of the ...

    The existence of bovine diarrhea viral nucleic acids in fetal bovine serum (FBS) was further confirmed by PCR methods (data not shown) [18], which may represent contamination of bovine serum used ...

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  • Anti-SOX4 antibody [154C4a] (ab70598)

    2021-7-29u2002·u2002ab70598 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

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  • Lipid Nanoparticle Formulation Increases Efficiency of DNA ...

    2020-5-29u2002·u2002Using 96 well plates, serum was heat inactivated (56 °C for 30 minutes) and diluted in MEM containing 5% heat inactivated fetal bovine serum (FBS). ZIKV was added to each well to a final ...

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  • NABERTHERM INC.

    Supplier: NABERTHERM INC. Description: The chamber furnaces LH 15/12 - LF 120/14 have been trusted for many years as professional chamber furnaces for the laboratory.,75994-612EA,75993-884EA,75994-624EA,75993-912EA,75994-608EA,75993-894EA,75993-878EA. This product is …

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  • Eulophia macrobulbon – an orchid with significant anti ...

    2017-1-15u2002·u2002Purpose. This study was designed to elucidate the anti-inflammatory, antioxidant and cytotoxic potential of a 50% ethanolic extract of Eulophia macrobulbon roots (EME) in vitro, an orchid growing in Southern Asia.Furthermore, the main active compounds were isolated, and the bioactivity of the single constituents was determined.

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  • GMP Oligonucleotides for IVD and ASR Applications ...

    GMP Oligonucleotides for IVD and ASR Applications. Custom cGMP services with highly standardised process controls to support pharmaceutical drug development process as it advances through clinical trials and on to commercial production. Carola Grimminger, Marketing Manager Eurofins Genomics, [email protected]

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  • Residual Solvents Testing - Eurofins Scientific

    USP Residual Solvents <467> is separated into three classes based on their potential toxicity level. Class 1 residual solvents are known to cause unacceptable toxicities. Class 2 residual solvents are associated with less severe toxicities and Class 3 residual solvents are considered the least toxic. Testing should be performed for those ...

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  • Optimized purification strategies for the...

    2015-5-29u2002·u2002Fetal bovine serum (FBS) is widely used in tissue-culture medium to provide essential proteins, nutrients and other uncharacterized factors for optimum cell growth; however, the presence of bovine IgG (bIgG) in the serum (up to 50 mg/L) is the main source of …

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  • STEMCELL Technologies

    STEMCELL Technologies develops cell culture media, cell separation systems, instruments and other reagents for use in life sciences research.

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  • Improvements in the Oral Absorption and Anticancer ...

    2020-10-9u2002·u2002Caco-2 or MDCK cells (ATCC ® CCL-34™) were seeded at a density of 5×10 3 cells/well in 100 µL of DMEM containing 10% (v/v) fetal bovine serum and 1% penicillin/streptomycin. After cells had been incubated at 37°C for 24 h, they were treated with 100 µL of OP, OP/DLM, and ODSF in free DMEM (equivalent to 1, 10, 25, 50, 100, 250, and 500 ...

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  • Cell Surface Marker Mediated Purification of iPS Cell ...

    Prepare iPS cell medium: Supplement 500 ml Knockout DMEM media with 75 ml fetal bovine serum (FBS), 5 ml L-glutamine, 5 ml Non-essential amino acids, 500 µl β-Mercaptoethanol, 5 x 10 5 units Leukaemia inhibitory factor (LIF). Please refer to Materials List for product and purchasing information for reagents used in the context of this manuscript.

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  • EZ-PCR Mycoplasma Kit for the detection of mycoplasma

    Product Overview: The EZ-PCR™ Mycoplasma Detection Kit is a highly-sensitive and -specific PCR assay designed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures with a detection limit of 10 CFU/mL. The kit contains a ready-to-use PCR reaction mix with highly-optimized mycoplasma-specific primers, a positive control, and an internal ...

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  • NABERTHERM INC.

    Supplier: NABERTHERM INC. Description: The chamber furnaces LH 15/12 - LF 120/14 have been trusted for many years as professional chamber furnaces for the laboratory.,75994-612EA,75993-884EA,75994-624EA,75993-912EA,75994-608EA,75993-894EA,75993-878EA. This product is …

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  • Structural Basis for Neutralization and Protection by a ...

    2019-3-19u2002·u2002HEK293T, vero and C6/36 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) (GIBCO) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (GIBCO) and Penicillin/Streptonmycin (macgene). HEK293T andvero cells were cultured at 37°C while C6/36 cells at 29°C. Viruses. ZIKV GZ01 strain was used for all studies.

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    Avantor ®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries.Our portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve.

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    Supplier: CRESS MFG CO INC Description: Model AC136/PM3T is a heat treating furnace used for treating metals with a maximum temperature of up to 2250°F,80701-374EA

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  • Optimized purification strategies for the...

    2015-5-29u2002·u2002Fetal bovine serum (FBS) is widely used in tissue-culture medium to provide essential proteins, nutrients and other uncharacterized factors for optimum cell growth; however, the presence of bovine IgG (bIgG) in the serum (up to 50 mg/L) is the main source of …

    Get Price
  • Improvements in the Oral Absorption and Anticancer ...

    2020-10-9u2002·u2002Caco-2 or MDCK cells (ATCC ® CCL-34™) were seeded at a density of 5×10 3 cells/well in 100 µL of DMEM containing 10% (v/v) fetal bovine serum and 1% penicillin/streptomycin. After cells had been incubated at 37°C for 24 h, they were treated with 100 µL of OP, OP/DLM, and ODSF in free DMEM (equivalent to 1, 10, 25, 50, 100, 250, and 500 ...

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  • (PDF) A Single Amino Acid Substitution in the Envelope ...

    PFU/0.5 ml in minimal essential medium containing 50% fetal bovine serum [FBS]) of one of three viruses via s.c. injection as follows: group 1, ChimeriVax-DEN1 uncloned virus (Vero P4 [M39R, E204R ...

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  • A novel multistage antiplasmodial inhibitor targeting ...

    2020-12-11u2002·u2002The mammalian cell lines HepG2 and 293T were cultured in 10-cm dishes at 37 °C and 5% CO 2 with Dulbecco's Modified Eagle Medium consisting of 10% fetal bovine serum and 1% penicillin ...

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  • Structural Basis for Neutralization and Protection by a ...

    2019-3-19u2002·u2002HEK293T, vero and C6/36 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) (GIBCO) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (GIBCO) and Penicillin/Streptonmycin (macgene). HEK293T andvero cells were cultured at 37°C while C6/36 cells at 29°C. Viruses. ZIKV GZ01 strain was used for all studies.

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  • EZ-PCR Mycoplasma Kit for the detection of mycoplasma

    Product Overview: The EZ-PCR™ Mycoplasma Detection Kit is a highly-sensitive and -specific PCR assay designed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures with a detection limit of 10 CFU/mL. The kit contains a ready-to-use PCR reaction mix with highly-optimized mycoplasma-specific primers, a positive control, and an internal ...

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  • NABERTHERM INC.

    Supplier: NABERTHERM INC. Description: The chamber furnaces LH 15/12 - LF 120/14 have been trusted for many years as professional chamber furnaces for the laboratory.,75994-612EA,75993-884EA,75994-624EA,75993-912EA,75994-608EA,75993-894EA,75993-878EA. This product is …

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  • Single-Sample Osmometer, Model 3250, Advanced ... - VWR

    3250 3250. 12000-630EA 28766.23 USD. CA12000-630 12000-630. Single-Sample Osmometer, Model 3250, Advanced Instruments. Osmometers. This single-sample osmometer utilizes the freezing-point depression method with 0.25mL samples, making it ideal for many clinical and industrial applications. Other features include multi-language capability (choose ...

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  • Ovens

    Supplier: CRESS MFG CO INC Description: Model AC136/PM3T is a heat treating furnace used for treating metals with a maximum temperature of up to 2250°F,80701-374EA

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    The Powerpette Plus Pipet Controller is a compact and portable device that features multiple user-selectable modes and speeds to perform a wide range of liquid handling applications.Pipette controllers rapidly fill and release accurate volumes from a wide range of glass or plastic blow-out pipettes. The ambidextrous devices offer different function modes and changeable speeds to adjust to ...

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  • Different Changes in Protein and Phosphoprotein Levels ...

    The current study compares five high-grade glioma and seven adenocarcinoma cell lines to determine the impact of serum starvation (simulated by culture in 0.5% fetal bovine serum [FBS]) on protein and phosphoprotein signals between the two …

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  • TranslatingResearchintoClinicalScaleManufacturingof ...

    2019-7-31u2002·u2002serum (HS) share characteristics with cells cultivated in fetal bovine serum (FBS) [22, 26]. Although all criteria ... system for establishments that manufacture human cells, tissues, and cellular and tissue-based products (HCT/P's) and to establish donor-eligibility, current good …

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  • IVF culture media: past, present and future

    The team also managed to cultivate human embryos up to the blastocyst stage using Ham's F10 with human or fetal calf serum (Steptoe et al., 1971). The embryos from the first IVF cycles were cultured in Earle's simple salt solution with pyruvate supplemented with the patient's serum, and the first IVF baby was born ( Steptoe and Edwards, 1978 ).

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  • (PDF) Serum and Protein Free Media - ResearchGate

    Serum and Protein Free Media. Michael Butler. Abstract For many decades bovine serum has been used as an essential compo-. nent for the growth of animal cells …

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  • A novel multistage antiplasmodial inhibitor targeting ...

    2020-12-11u2002·u2002The mammalian cell lines HepG2 and 293T were cultured in 10-cm dishes at 37 °C and 5% CO 2 with Dulbecco's Modified Eagle Medium consisting of 10% fetal bovine serum …

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    2011-5-18u2002·u2002HeLa pLuc 705 cells, kindly provided by R. Kole and B. Lebleu were grown at 37°C, 5% CO 2 in Dulbecco's Modified Eagle's Medium (DMEM) with glutamax supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, 10% fetal bovine serum, 100 U/ml penicillin and 100 mg/ml streptomycin (PAA Laboratories GmbH, Germany).

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  • Structural Basis for Neutralization and Protection by a ...

    2019-3-19u2002·u2002The crystal structure of the FabZK2B10-DIII complex was characterized to a resolution of 2.32 Å (Figure 1; Table S1).Two FabZK2B10-DIII heterodimers are found in the asymmetric unit, with only minor differences in the constant domains of the two FabZK2B10s ().The DIII of ZIKV E protein adopts an elongated immunoglobulin-like fold with two layers of β strands that are …

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  • Pharmacological influences of natural products as ...

    2018-7-24u2002·u2002Popularity of herbal remedies is increasing day by day despite the presence of synthetic drugs to treat the Liver Diseases owing to the adverse effects and high cost of synthetic drugs. Silymarin has tremendous potential for the treatment of various liver disorders because of its high antioxidant potential as liver diseases are associated with increased oxidative stress.

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  • Single-Sample Osmometer, Model 3250, Advanced ... - VWR

    3250 3250. 12000-630EA 28766.23 USD. CA12000-630 12000-630. Single-Sample Osmometer, Model 3250, Advanced Instruments. Osmometers. This single-sample osmometer utilizes the freezing-point depression method with 0.25mL samples, making it ideal for many clinical and industrial applications. Other features include multi-language capability (choose ...

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    2021-4-27u2002·u2002Best hair loss treatments for 2021. Regenerative medicine for hair loss patients includes high-quality lysed platelets, prescriptions for topical finasteride with minoxidil, dutasteride, amniotic membrane, adipose-derived stem cells, Acell, appropriate vitamins, whey protein, low-level laser caps, and other nutritional supplements.

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  • Spatiotemporal Dynamics and Epistatic Interaction Sites in ...

    2013-9-9u2002·u2002The samples were cultured in C6/36 cells and maintained at 28°C in Delbecco modified Eagle medium (DMEM, Gibco-BRL, Gaithersbury, MD) supplemented with 2% fetal bovine serum (Gibco-BRL). The inoculated cells were harvested and identified by using a direct immunofluorescence antibody.

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  • Structural Basis for Neutralization and Protection by a ...

    2019-3-19u2002·u2002HEK293T, vero and C6/36 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) (GIBCO) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (GIBCO) and Penicillin/Streptonmycin (macgene). HEK293T andvero cells were cultured at 37°C while C6/36 cells at 29°C. Viruses. ZIKV GZ01 strain was used for all studies.

    Get Price
  • Eulophia macrobulbon – an orchid with significant anti ...

    2017-1-15u2002·u2002Purpose. This study was designed to elucidate the anti-inflammatory, antioxidant and cytotoxic potential of a 50% ethanolic extract of Eulophia macrobulbon roots (EME) in vitro, an orchid growing in Southern Asia.Furthermore, the main active compounds were isolated, and the bioactivity of the single constituents was determined.

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  • Laboratory evaluation of molecular...

    2019-12-19u2002·u2002Extraction of DNA from mosquitoes and tsetse flies. In preparation for DNA isolation, 20 μL of Proteinase K, 180 μL of Buffer ATL and a 4.5 mm ball bearing were added to all carcass and midgut samples. Samples were then mechanically homogenized at a setting of 30.0 1/S for 5 min using a TissueLyser II (Qiagen).

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  • Two Key Residues in EphrinB3 Are Critical for Its Use as ...

    2006-2-10u2002·u2002EphrinB2 was recently discovered as a functional receptor for Nipah virus (NiV), a lethal emerging paramyxovirus. Ephrins constitute a class of homologous ligands for the Eph class of receptor tyrosine kinases and exhibit overlapping expression patterns. Thus, we examined whether other ephrins might serve as alternative receptors for NiV. Here, we show that of all …

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  • Cell Imaging - Promega

    Replace the 1X PBS with an equal volume of PBS + 2% donkey serum + 0.01% Triton® X-100, and block for 1 hour at room temperature. Dilute the Anti-HaloTag® pAb 1:500 in PBS + 1% donkey serum, to final labeling concentration of 2μg/ml. Replace the blocking solution with the antibody solution, and incubate for 1 hour at room temperature.

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  • IDM Members Meeting Dates 2021

    2021-2-10u2002·u2002IDM Members' meetings for 2021 will be held from 12h45 to 14h30.A zoom link or venue to be sent out before the time.. Wednesday 10 February; Wednesday 5 May; Wednesday 4 August; Wednesday 3 November

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  • Cellular agriculture in the UK: a review

    2020-1-24u2002·u2002Competing interests: NS is a founding member of Cultivate, a third sector multi-voiced forum for discussing cellular agriculture from UK perspectives, and this publication forms part of the work of Cultivate. ME is a founding member of Cultivate, a third sector multi-voiced forum for discussing cellular agriculture from UK perspectives, and this publication forms part of the work …

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  • Institute Of Infectious Disease and Molecular Medicine

    2021-10-2u2002·u2002For information on South Africa's response to COVID-19 please visit the COVID-19 Corona Virus South African Resource Portal.

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  • tipped pcbn inserts in 60 degree triangle shape T for hard ...

    2021-7-20u2002·u2002tipped pcbn inserts in 60 degree triangle shape T for hard turning ferrous metals of cast iron and hardened steel, the cbn insert cutting edges are made with polycrystalline cubic boron nitride, indexable inserts with cbn tips are precision cutting tools, which are used in cnc fine finish machining and turning roller, bearing, pumps, automobile brake disk, aircraft jet engine.

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    inactivation as manufacturers have to undertake different tests for batches destined for different markets. Test for inactivation should include a direct test in mice and an indirect test by amplification on cell substrates. The EP permits the use of immunofluorescence for the detection of virus in cell culture whereas the WHO

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  • Frontiers

    Friedenstein et al. (1970) were the first to report the culture of fibroblast-like colonies from guinea pig bone marrow in media supplemented with fetal bovine serum (FBS). Media supplemented with 10–20% FBS has since been recognized as a conventional method to expand hMSCs from various tissue sources (Haynesworth et al., 1992) and has been used as an ancillary reagent in clinical trials ...

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  • Spatiotemporal Dynamics and Epistatic Interaction Sites in ...

    2013-9-9u2002·u2002Introduction. An alarming trend in the epidemiological profile of dengue virus (DENV) in recent decades is the dramatic increases in its morbidity and mortality rates and the correlation of its geographic distribution with climate change and globalisation [1,2].In 2007, the Intergovernmental Panel on Climate Change reported that the mean global temperature has increased by roughly 0.74°C ± 0 ...

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    2020-4-20u2002·u2002Human keratinocytes and derived products are crucial for skin repair and regeneration. Despite substantial advances in engineered skin equivalents, their poor availability and immunorejection remain major challenges in skin grafting. Induced keratinocyte-like cells (iKCs) were directly reprogrammed from human urine cells by retroviral transduction of two lineage-specific transcription …

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  • Cell Imaging - Promega

    Replace the 1X PBS with an equal volume of PBS + 2% donkey serum + 0.01% Triton® X-100, and block for 1 hour at room temperature. Dilute the Anti-HaloTag® pAb 1:500 in PBS + 1% donkey serum, to final labeling concentration of 2μg/ml. Replace the blocking solution with the antibody solution, and incubate for 1 hour at room temperature.

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  • Ferritin - dpcweb.com

    2016-4-17u2002·u2002seen in the pattern of serum ferritin values under a variety of physiological and pathological conditions. For individuals in good health the median level, slightly elevated at birth, reaches a low of about 30 ng/mL at six months, with the increase to adult levels taking place after puberty.7 Hodgkin's disease and certain other

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  • tipped pcbn inserts in 90 degree square shape S for hard ...

    2021-7-20u2002·u2002tipped pcbn inserts in 90 degree square shape S for hard turning ferrous metals of cast iron and hardened steel, the cbn insert cutting edges are made with polycrystalline cubic boron nitride, indexable inserts with cbn tips are precision cutting tools, which are used in cnc fine finish machining and turning roller, bearing, pumps, automobile brake disk, aircraft jet engine.

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  • Trikoot's Milking Machine Buying Guide – Trikoot

    Previously known as Venus 2000, and manufactured by Syb Inc. (doing business as ABCO Research Associates and Sybian) the Venus for Men milking machine is a self-contained, single-tube milking machine. Due to its design, the Venus for Men is easy to use and quick to set up. It's almost silent in operation and will not disturb your neighbours.

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  • Institute Of Infectious Disease and Molecular Medicine

    2021-10-2u2002·u2002For information on South Africa's response to COVID-19 please visit the COVID-19 Corona Virus South African Resource Portal.

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  • Darshan Gandhi - Assistant General Manager - Serum ...

    Assistant General Manager. Serum Institute of India private limited. Oct 2003 - Present17 years 5 months. Pune Area, India. Presently working at, serum institute of India limited as AGM. I am leading group of 27 officers and 18 workers involved in commercial. production of Hib polysaccharide @. 800 L scale .

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