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    TeSR™-E7™ yields higher reprogramming efficiencies in feeder-free conditions compared to other media in both low and high passage NHDF. Low (p3) and high passage (p7) NHDF were reprogrammed as described in either TeSR™-E7™ or other media, and the number of ES-like colonies were counted after 28 days.

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    ClonaCell™-CHO ACF Supplement is a serum-free and animal component-free supplement that is added to protein-free media in order to increase cloning efficiency and promote robust growth of CHO cells. The 40X supplement can be added to a variety of protein-free liquid or semi-solid cell culture media to increase the growth and single-cell ...

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    MethoCult™ H4330 is a methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. This formulation contains erythropoietin (EPO), but does not contain other cytokines.

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    2020-1-1u2002·u2002Animal cell culture has found use in diverse areas, from basic to advanced research. It has provided a model system for various research efforts: 1. The study of basic cell biology, cell cycle mechanisms, specialized cell function, cell–cell and cell–matrix interactions. 2.

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    MethoCult™ H4435 Enriched (MethoCult™ GF+ H4435) is a complete methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, …

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    B. a hydrogen bond forms between sodium and chlorine. C. sodium and chlorine share electrons to form a bond. D. chlorine gives an electron sodium. E. sodium crystals combine with chlorine crystals. none of the statements are true. The body uses atoms in different ways to accomplish different tasks. For example, one portion of the body's calcium ...

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    2021-9-18u2002·u2002Bio-artificial liver biomass cryopreservation with different systems for post-thaw culture presented at Society of Low Temperature Biology meeting.

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  • MethoCult™ H4330 Methylcellulose-Based Medium for

    MethoCult™ H4330 is a methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. This formulation contains erythropoietin (EPO), but does not contain other cytokines.

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  • MethoCult™ H4435 Enriched Methylcellulose Medium

    MethoCult™ H4435 Enriched (MethoCult™ GF+ H4435) is a complete methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, …

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    Igelmann et al. identified a hydride transfer complex (HTC) that drives metabolic reprogramming to overcome senescence and induces tumorigenesis. HTC consists of pyruvate carboxylase, malate dehydrogenase 1 and malic enzyme 1 and catalyzes a metabolic cycle whose net effect is to supply NAD+ and NADPH, which are key cofactors for many essential metabolic reactions.

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    2002-8-1u2002·u2002Nutrient-hormone interaction in the ovine liver: methionine supply selectively modulates growth hormone-induced IGF-I gene expression August 2002 Journal of …

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  • Animal tissue culture principles and applications ...

    2020-1-1u2002·u2002Animal cell culture has found use in diverse areas, from basic to advanced research. It has provided a model system for various research efforts: 1. The study of basic cell biology, cell cycle mechanisms, specialized cell function, cell–cell and cell–matrix interactions. 2.

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    2020-4-6u2002·u2002SARS-CoV-2 belongs to the new coronavirus species in the genus containing SARS-CoV and MERS-CoV [].It is enveloped, and the particles are round or elliptic, usually pleomorphic, with a diameter of 60~140 nm [16, 17].Its genetic characteristics are different from those of SARS-CoV and MERS-CoV [].Current studies have shown that SARS-like coronavirus in bat (bat-SL …

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    ZenBio Antioxidant Assay Kits can be used to determine the total antioxidant capacity of biological fluids, cells, and tissue. They can also be used to assay the antioxidant activity of naturally occurring or synthetic compounds for use as dietary supplements, topical protection, and therapeutics. Cosmeceutical Kits ZenBio Biological Products and Services

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  • COMPOSITIONS AND METHODS FOR TREATING ALPHA-1

    1. (canceled) 2. A method of expressing AAT in a subject in need thereof, comprising administering: i) a nucleic acid construct comprising a heterologous AAT protein coding sequence; ii) a RNA-guided DNA binding agent; and iii) an albumin guide RNA (gRNA) comprising a sequence chosen from: a) a sequence that is at least 95%, 90%, 85%, 80%, or 75% identical …

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    FIGS. 27A-C show liver TTR editing (FIG. 27A) and serum TTR results (in μg/mL (FIG. 27B) and as percentage of TSS-treated control (FIG. 27C)), respectively, from humanized TTR mice dosed with LNP formulations across a range of doses with guides G000481, G000482, G000486 and G000499 and containing Cas9 mRNA (SEQ ID NO: 1) in a 1:1 ratio by ...

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    2021-5-22u2002·u2002The liver fluke Fasciola gigantica secretes excretory-secretory proteins during infection to mediate its interaction with the host. In this study, we investigated the immunomodulatory effects of a recombinant tegumental calcium-binding EF-hand protein 4 of F. gigantica (rFg-CaBP4) on goat monocytes. The rFg-CaBP4 protein was induced and purified by …

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    2021-9-26u2002·u2002The team concluded that successful cryopreservation of encapsulated liver cell spheroids on a large scale, at 16-48% of a total human liver size (the biomass of 30% liver cell numbers is the likely requirement for such a bio-artificial liver) represented the correct order of magnitude and provided sufficient post-thaw recovery.

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    Grow HL-60 cells in RPMI-1640 medium containing 10% fetal bovine serum in a humidified 5% CO 2 incubator at 37°C. Adjust the cell density to 5 × 10 5 cells/ml and treat with anisomycin at a final concentration of 2μg/ml (dissolved in DMSO). Incubate for 2 hours in a humidified 5% CO 2 incubator at 37°C. Treat negative control cells with an ...

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  • PCR Amplification

    Introduction to PCR. The polymerase chain reaction (PCR) is a relatively simple technique that amplifies a DNA template to produce specific DNA fragments in vitro. Traditional methods of cloning a DNA sequence into a vector and replicating it in a living cell often require days or weeks of work, but amplification of DNA sequences by PCR ...

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  • Initial success in the identification and management of ...

    2020-4-6u2002·u2002SARS-CoV-2 belongs to the new coronavirus species in the genus containing SARS-CoV and MERS-CoV [].It is enveloped, and the particles are round or elliptic, usually pleomorphic, with a diameter of 60~140 nm [16, 17].Its genetic characteristics are different from those of SARS-CoV and MERS-CoV [].Current studies have shown that SARS-like coronavirus in bat (bat-SL …

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    1994-1-1u2002·u2002Also in 1975, Goldstein found the presence of a peptide-like substance in the bovine pituitary gland with opioid activity (Cox el al., 1975). This substance was a polypeptide with 31 amino acids and its action could be antagonized with naloxone as well. Hughes and their co-workers named the pentapeptides leucine enkaphalin and methionine ...

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    2021-9-18u2002·u2002Bio-artificial liver biomass cryopreservation with different systems for post-thaw culture presented at Society of Low Temperature Biology meeting.

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  • Nutrient-hormone interaction in the ovine liver ...

    2002-8-1u2002·u2002Nutrient-hormone interaction in the ovine liver: methionine supply selectively modulates growth hormone-induced IGF-I gene expression August 2002 Journal of Endocrinology 174(2):335-41

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  • Differential Gene Expression in Liver, Gill, and Olfactory ...

    2015-8-11u2002·u2002Most Pacific salmonids undergo smoltification and transition from freshwater to saltwater, making various adjustments in metabolism, catabolism, osmotic, and ion regulation. The molecular mechanisms underlying this transition are largely unknown. In the present study, we acclimated coho salmon (Oncorhynchus kisutch) to four different salinities and assessed gene expression through …

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    2016-1-1u2002·u2002Cells of the liver. Hepatocytes (referred to as parenchymal cells) constitute ~70-80% of the liver mass.However, >50% of liver DNA is found in smaller nonparenchymal cells (bile duct epithelium, hepatic stellate cells, sinusoidal endothelium, Kupffer cells) and itinerant cells (such as leukocytes). The hepatocyte is a polygonal epithelial cell, ~30-40 µm in diameter, arranged in single-cell ...

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  • Differentiation of human pluripotent stem cells into two ...

    2018-4-3u2002·u2002The cells were permeabilized for 15 min with 0.2% Triton X-100 (Sigma, USA) in PBS (PBST), and blocked for at least 2 h with 6% bovine serum albumin (BSA) in PBST at room temperature. The cells were then incubated at 4 °C overnight with the primary antibodies as described in Table Table2. 2. The cells were washed three times with tris-buffered ...

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  • Initial success in the identification and management of ...

    2020-4-6u2002·u2002SARS-CoV-2 belongs to the new coronavirus species in the genus containing SARS-CoV and MERS-CoV [].It is enveloped, and the particles are round or elliptic, usually pleomorphic, with a diameter of 60~140 nm [16, 17].Its genetic characteristics are different from those of SARS-CoV and MERS-CoV [].Current studies have shown that SARS-like coronavirus in bat (bat-SL-CoVZC45, MG772933.1) has …

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    2019-5-9u2002·u2002An increase in rat liver weights at 100 mg/kg/day in the 2 and 13 week studies and 70 mg/kg/day in the 26 week study was associated with minimally to mildly higher bilirubin concentrations (0.6 to 0.7 mg/dL versus 0.1 mg/dL in controls), which was likely to be associated with organic anion transporting polypeptide inhibition or with the ...

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  • PCR Amplification

    Introduction to PCR. The polymerase chain reaction (PCR) is a relatively simple technique that amplifies a DNA template to produce specific DNA fragments in vitro. Traditional methods of cloning a DNA sequence into a vector and replicating it in a living cell often require days or weeks of work, but amplification of DNA sequences by PCR ...

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  • COMPOSITIONS AND METHODS FOR TREATING ALPHA-1

    1. (canceled) 2. A method of expressing AAT in a subject in need thereof, comprising administering: i) a nucleic acid construct comprising a heterologous AAT protein coding sequence; ii) a RNA-guided DNA binding agent; and iii) an albumin guide RNA (gRNA) comprising a sequence chosen from: a) a sequence that is at least 95%, 90%, 85%, 80%, or 75% identical to a sequence selected from the group ...

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  • Bio-artificial liver biomass cryopreservation with ...

    2021-9-18u2002·u2002Bio-artificial liver biomass cryopreservation with different systems for post-thaw culture presented at Society of Low Temperature Biology meeting.

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  • Potential lactoferrin activity against pathogenic viruses ...

    2014-10-1u2002·u2002The LF sequences of nine species are currently known. These are LFs from human, bovine, camel, buffalo, sheep, goat, pig, horse, and mouse. It has been emphasized that there is a noticeable range in the LF pair-wise sequence identities .This fact is illustrated by Fig. 2 where the pair-wise sequence identities of different LFs are shown. This analysis shows that the pair-wise …

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  • Sexual Differentiation - Endotext - NCBI Bookshelf

    2020-5-27u2002·u2002The chromosomal sex of the embryo is established at fertilization. However, 6 weeks elapse in humans before the first signs of sex differentiation are noticed. Sex differentiation involves a series of events whereby the sexually indifferent gonads and genitalia progressively acquire male or female characteristics. Believed initially to be governed entirely by the presence or absence of …

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  • Evolution of Hominin Detoxification: Neanderthal and ...

    Map of the modern human and ancient hominin AHR mRNA sequence variants studied here and by Hubbard et al. (2016).The modern human AHR variant tested was based on manually expert-curated sequence information from the Ensembl and RefSeq databases (details in Materials and Methods). The bar gives a scaled representation of this 6,277-bp sequence including the 5′-UTR, the AHR …

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  • Differential Gene Expression in Liver, Gill, and Olfactory ...

    2015-8-11u2002·u2002Most Pacific salmonids undergo smoltification and transition from freshwater to saltwater, making various adjustments in metabolism, catabolism, osmotic, and ion regulation. The molecular mechanisms underlying this transition are largely unknown. In the present study, we acclimated coho salmon (Oncorhynchus kisutch) to four different salinities and assessed gene …

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  • Premature Infants and Breastfeeding - ScienceDirect

    Mother's own milk, appropriately fortified, is the standard for feeding premature infants providing short- and long-term health benefits. Neonatal providers play an important role supporting lactation in the neonatal intensive care unit with education, milk …

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    Grammarly keeps security at the heart of our product, infrastructure, and company operations. Learn more about our security practices.Grammarly's SOC 2 (Type 2) report attests to our enterprise-grade system and organization controls regarding security, …

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  • Differentiation of human pluripotent stem cells into two ...

    2018-4-3u2002·u2002The expression of a specific combination of transcription factors (TFs) in the multipotent progenitor cells (MPCs) is critical for determining pancreatic cell fate. NKX6.1 expression in PDX1+ MPCs is required for functional β cell generation. We have recently demonstrated the generation of a novel population of human pluripotent stem cell (hPSC)-derived MPCs that exclusively express …

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  • Animal Science Final Review Questions and Study Guide ...

    trace minerals, meaning they are present at low levels in the body or required in smaller amounts in the animals diet. _______ include chromium, cobalt, copper, fluorine, iodine, iron, manganese, molybdenum, selenium, and zinc. Megacalorie. the energy needed to raise the temperature of 1 megagram of water through 1 °C.

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  • Premature Infants and Breastfeeding - ScienceDirect

    Mother's own milk, appropriately fortified, is the standard for feeding premature infants providing short- and long-term health benefits. Neonatal providers play an important role supporting lactation in the neonatal intensive care unit with education, milk expression, skin-to …

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  • Differentiation of human pluripotent stem cells into two ...

    2018-4-3u2002·u2002The cells were permeabilized for 15 min with 0.2% Triton X-100 (Sigma, USA) in PBS (PBST), and blocked for at least 2 h with 6% bovine serum albumin (BSA) in PBST at room temperature. The cells were then incubated at 4 °C overnight with the primary antibodies as described in Table Table2. 2. The cells were washed three times with tris-buffered ...

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  • Differentiation of human pluripotent stem cells into two ...

    2018-4-3u2002·u2002The expression of a specific combination of transcription factors (TFs) in the multipotent progenitor cells (MPCs) is critical for determining pancreatic cell fate. NKX6.1 expression in PDX1+ MPCs is required for functional β cell generation. We have recently demonstrated the generation of a novel population of human pluripotent stem cell (hPSC) …

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    The analysis of the state of the potato industry in the Lower Volga region is made. The heat and moisture supply and soil conditions of the region and their compliance with the biological characteristics of potato plants are estimated. The object of research is the planting of potatoes of promising varieties under drip irrigation.

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    Hepatosplenic schistosomal infection is a common parasitic liver disease in Egypt, resulting in the formation of granuloma followed by fibrosis which leads to the chronic liver disease.

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    Liver diseases are extremely costly in terms of human suffering, general practitioner and hospital visits, and premature loss of productivity. Most people with liver disease die in working age (16-85 years) making liver disease the third biggest cause of premature mortality, mortality rates for liver disease have increased 400% since 1970.

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    Request PDF International Union of Pharmacology. LXII. The NR1H and NR1I Receptors: Constitutive Androstane Receptor, Pregnene X Receptor, Farnesoid X Receptor, Farnesoid X Receptor beta, Liver ...

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  • Animal Science Final Review Questions and Study Guide ...

    trace minerals, meaning they are present at low levels in the body or required in smaller amounts in the animals diet. _______ include chromium, cobalt, copper, fluorine, iodine, iron, manganese, molybdenum, selenium, and zinc. Megacalorie. the energy needed to raise the temperature of 1 megagram of water through 1 °C.

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