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Fetal Bovine Serum/ Fetal Bovine Serum - The CHMP/CVMP Note for Guidance on minimising the risk of transmitting animal spongiform encephalopathy agents via human and veterinary medicinal products (?TSE Note for Guidance?, ref. 1) recommends that when a manufacturer has a choice between the use of ruminant or non-ruminant materials, the use of non-ruminant …
Get Price2021-2-12u2002·u2002Fat mass can also be lowered by mobilising accumulated TG stores through lipolysis to fatty acids and glycerol that are then metabolised or lost to the medium. Treatment of cells with the lipolysis protocol for just 24 h reduced fat content with the higher dMF concentrations (Fig. 7 C) without adversely affected cell viability (Fig. 7 D).
Get Price2018-6-7u2002·u2002The cellular TG content was measured enzymatically with a commercial Fuji Dri-Chem 3500 kit (Fujifilm, Tokyo, Japan) following the manufacturer's instructions. The TG content was expressed in micrograms of TGs per microgram of protein. Each treatment was performed in triplicate. 2.5. Animals and Treatment
Get Price2016-8-16u2002·u2002The efficiency of maltodextrin, whey protein isolate, and pea protein isolate to formulate a polyphenol-enriched grape marc extract by spray drying has been compared. Different inlet (120–140 °C ) and outlet (81–89 °C ) temperatures have been evaluated, as well as the amount of carrier expressed as carrier/extract ratio (0.1:1–1:1). The particles obtained were …
Get PriceNon-alcoholic fatty liver disease (NAFLD) is a common metabolic disorder that causes excess lipid accumulation in the liver and is the leading cause of end-stage liver disease. Liriope platyphylla is a medicinal herb that has long been used to treat cough, obesity, and diabetes. However, the effect of Liriope platyphylla on NAFLD has not been studied. The aim of this …
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Get Price2018-7-31u2002·u2002Extract (d) was inoculated with A. oryzae NK, incubated at 30 °C for 36 h, and sterilized at 95 °C for 1 h (e). After filtration, the mixture was concentrated (f) and sterilized (95 °C, 2 h) to obtain extract (g). After completion of the spray-drying process, the final sample powder (h; fSBE) was prepared. Extracts were stored at − 80 °C ...
Get PriceLow Molecular Weight 90% Fish Collagen Powder Granule . Our Fish Collagen is widely used in below formulated products: 1. Used for Joint health dietary Supplements: together with other joint heatlh ingredients such as chondroitin sulfate, glucosamine, hyaluronic acid (sodium hyaluronate) and Curcumin extract Powder.
Get Price2018-6-16u2002·u2002Japan, and zicai in China, has increased in demand because of the increasing popularity of Oriental cuisine and macrobiotic diets in western countries in recent years.2,3) From a nutritional point of view, lavers are characterized by high concentrations of fibers and min-erals,4,5) low fat content, and relatively high protein
Get Price2019-7-31u2002·u2002Extract of Wax Gourd Peel Prevents High-Fat Diet-Induced Hyperlipidemia in C57BL/6 Mice via the Inhibition of the ... supplemented with% fetal bovine serum (FBS, Hyclone, Logan, UT), units/mL penicillin, ... Liver Lipid Content Analysis. e liver tissues were weighed and homogenized in lysis bu er (mM Tris-HCl ...
Get Price2016-8-16u2002·u2002The efficiency of maltodextrin, whey protein isolate, and pea protein isolate to formulate a polyphenol-enriched grape marc extract by spray drying has been compared. Different inlet (120–140 °C ) and outlet (81–89 °C ) temperatures have been evaluated, as well as the amount of carrier expressed as carrier/extract ratio (0.1:1–1:1). The particles obtained were …
Get Price636 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 39: 636-644, 2017 Abstract. In this study, we examined the inhibitory effects of enzyme treated - Ecklonia cava (EEc) extract on the adipogen- esis of 3T3-L1 adipocytes. The components of Ecklonia cava (E. cava) were first separated and purified using the diges- tive enzymes pectinase (Rapidase® X-Press L) and …
Get Price2018-7-31u2002·u2002Extract (d) was inoculated with A. oryzae NK, incubated at 30 °C for 36 h, and sterilized at 95 °C for 1 h (e). After filtration, the mixture was concentrated (f) and sterilized (95 °C, 2 h) to obtain extract (g). After completion of the spray-drying process, the final sample powder (h; fSBE) was prepared. Extracts were stored at − 80 °C ...
Get Price2018-12-8u2002·u2002In recent years, obesity has become a key factor affecting human health. Moringa oleifera Lam. is a perennial tropical deciduous tree, which is widely used in human medicine due to its nutritional and unique medicinal value. It has a cholesterol-lowering effect, but its mechanism of action is unclear. In this study, we elucidated the inhibitory effect of M. oleifera …
Get Price2020-3-17u2002·u2002Sample preparation. Standardized RGS and GG were purchased from Korean Ginseng Corporation (Daejeon, Korea). Standardized red ginseng extracts were fractionated with Diaion HP20 column chromatography using H 2 O, 30% ethanol (EtOH), and 95% EtOH as eluents in a sequential elution process. Then 95% EtOH fraction was vacuum concentrated …
Get Price2016-9-1u2002·u20021. Introduction. Atherosclerosis is reported to be the main pathological cause of cardiovascular disease (CVD). Dyslipidemia, including higher serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) and lower high-density lipoprotein cholesterol (HDL-C), is a major risk factor for atherosclerosis .Dietary fat is considered one of the most important …
Get Pricesclerotium of P. cocos was ground into powder and extracted twice with 10 volumes of 80% ethanol at 85-90˚C in a reflux condenser for 3 h. After being filtered through a 0.2-µm filter, the extract was concentrated and lyophilized by vacuum evap - oration at 60˚C. The solid form of the extract was dissolved
Get Price2017-10-27u2002·u2002toxic and antigenotoxic dose-dependent,17 antioxidant4,18–20 and antitumoral effects.7,21,22 Mentha cervina L. (Lamiaceae) also known as Hart's penny-royal, is an aromatic herb, found mainly in Eurasia and Africa.
Get PriceThe mean droplet size of TENE powder and TENE were 492.2 nm and 323.6 nm, respectively, indicating a single spectrum of size distribution. The curcumin content of TENE powder was 3.3 μg/mg, which was 15 times lower than that of turmeric extract powder (Table 1) (equation 1).
Get PriceIn murine fetal liver, Jagged1 is expressed in cells surrounding vessels, whereas Notch1 is expressed in all cells . In the adult liver, Jagged1, Notch1, and the activated cytoplasmic domain of Notch, NICD, are all present in hepatocytes and bile duct cells.
Get Price2018-12-8u2002·u2002In recent years, obesity has become a key factor affecting human health. Moringa oleifera Lam. is a perennial tropical deciduous tree, which is widely used in human medicine due to its nutritional and unique medicinal value. It has a cholesterol-lowering effect, but its mechanism of action is unclear. In this study, we elucidated the inhibitory effect of M. oleifera leaf petroleum …
Get Price2021-8-5u2002·u2002Anticancer effect of A. vasica extract against PA 1 cell lines. PA 1 cell lines were grown in Dulbecco's modified Eagle's medium supplemented with fetal bovine serum (FBS), penicillin, and streptomycin. The cells were subcultured after trypsinization with 0.25% in 0.5 mM EDTA and were cultured under 5% CO 2 at 37 °C.
Get Price2019-7-31u2002·u2002resistance, triacylglycerol (TG), and total cholesterol content. Addition of % YPEE signi cantly reduced the weight gain and rise in liver fat content, serum triacylglycerol (TG), total cholesterol, and insulin resistance found in mice fed a high-fat diet (HFD).
Get Price2019-7-31u2002·u2002Extract of Wax Gourd Peel Prevents High-Fat Diet-Induced Hyperlipidemia in C57BL/6 Mice via the Inhibition of the ... supplemented with% fetal bovine serum (FBS, Hyclone, Logan, UT), units/mL penicillin, ... Liver Lipid Content Analysis. e liver tissues were weighed and homogenized in lysis bu er (mM Tris-HCl ...
Get Price2017-10-27u2002·u2002toxic and antigenotoxic dose-dependent,17 antioxidant4,18–20 and antitumoral effects.7,21,22 Mentha cervina L. (Lamiaceae) also known as Hart's penny-royal, is an aromatic herb, found mainly in Eurasia and Africa.
Get Pricesclerotium of P. cocos was ground into powder and extracted twice with 10 volumes of 80% ethanol at 85-90˚C in a reflux condenser for 3 h. After being filtered through a 0.2-µm filter, the extract was concentrated and lyophilized by vacuum evap - oration at …
Get Price2020-6-12u2002·u2002BackgroundDiabetic nephropathy (DN) is a serious complication of diabetes mellitus (DM) with limited treatment options. DN leads to progressive renal failure and accelerates rapidly into end-stage renal disease. Astragalus mongholicus Bunge and Panax notoginseng (Burkill) F.H. Chen formula (APF) is a traditional Chinese medicine (TCM) formula widely used to treat chronic …
Get Price2018-11-9u2002·u2002spread on a plastic trays and left for drying in a chemical hood until a constant weight was obtained. The dried seeds were grounded to a fine powder using a mortar and pestle with liquid nitrogen added to keep the powder frozen. The dried powder was flashed with nitrogen and stored at -80 oC until used. A known quantity (5 g) of dried papaya ...
Get Price2019-5-6u2002·u2002exceptional nutritional value, with low lipid content and large amounts of carbohydrates and proteins, in addition to essen-tial amino acids and fat-soluble vitamins (vitamins A, D, E and K).1 Moreover, mushrooms are increasingly attractive as func-tional foods and as potential sources for the development of new drugs.2
Get Price2021-8-5u2002·u2002Anticancer effect of A. vasica extract against PA 1 cell lines. PA 1 cell lines were grown in Dulbecco's modified Eagle's medium supplemented with fetal bovine serum (FBS), penicillin, and streptomycin. The cells were subcultured after trypsinization with 0.25% in 0.5 mM EDTA and were cultured under 5% CO 2 at 37 °C.
Get Price2018-12-8u2002·u2002In recent years, obesity has become a key factor affecting human health. Moringa oleifera Lam. is a perennial tropical deciduous tree, which is widely used in human medicine due to its nutritional and unique medicinal value. It has a cholesterol-lowering effect, but its mechanism of action is unclear. In this study, we elucidated the inhibitory effect of M. oleifera leaf petroleum ether ...
Get PriceThe mean droplet size of TENE powder and TENE were 492.2 nm and 323.6 nm, respectively, indicating a single spectrum of size distribution. The curcumin content of TENE powder was 3.3 μg/mg, which was 15 times lower than that of turmeric extract powder (Table 1) (equation 1).
Get Price2017-10-27u2002·u2002toxic and antigenotoxic dose-dependent,17 antioxidant4,18–20 and antitumoral effects.7,21,22 Mentha cervina L. (Lamiaceae) also known as Hart's penny-royal, is an aromatic herb, found mainly in Eurasia and Africa.
Get Price2019-7-31u2002·u2002resistance, triacylglycerol (TG), and total cholesterol content. Addition of % YPEE signi cantly reduced the weight gain and rise in liver fat content, serum triacylglycerol (TG), total cholesterol, and insulin resistance found in mice fed a high-fat diet (HFD).
Get Pricesclerotium of P. cocos was ground into powder and extracted twice with 10 volumes of 80% ethanol at 85-90˚C in a reflux condenser for 3 h. After being filtered through a 0.2-µm filter, the extract was concentrated and lyophilized by vacuum evap - oration at 60˚C. …
Get Price2016-9-13u2002·u2002KBH-1 is an herbal mixture of Saururus chinensis, Curcuma longa and Polygala tenuifolia. Each herb has been reported to have various pharmaceutical activities; however, the synergistic effect of this herbal composition on obesity has not yet been determined. We investigated the alleviation effect of KBH-1 and its possible molecular mechanism in obesity-induced hepatic steatosis and leptin ...
Get Price2020-6-12u2002·u2002BackgroundDiabetic nephropathy (DN) is a serious complication of diabetes mellitus (DM) with limited treatment options. DN leads to progressive renal failure and accelerates rapidly into end-stage renal disease. Astragalus mongholicus Bunge and Panax notoginseng (Burkill) F.H. Chen formula (APF) is a traditional Chinese medicine (TCM) formula widely used to treat chronic kidney diseases …
Get Price2021-9-29u2002·u2002Insulin resistance (IR), caused by impaired insulin signal and decreased insulin sensitivity, is generally responsible for the pathophysiology of type 2 diabetes mellitus (T2DM). Sesquiterpene glycosides (SGs), the exclusive natural products from loquat leaf, have been regarded as potential lead compounds owing to their high efficacy in hypoglycemia and hypolipidemia.
Get Price2020-4-8u2002·u2002The liver fat content reflects lipogenesis (Adiels et al., 2006), and hepatic tissue weight is an important target for the anti‐obesity effect. The MF‐treated group showed decreased liver weight, when compared with the HFD group.
Get PriceThe cells were cultured in Dulbecco's modified Eagle's medium (DMEM), containing 10% fetal bovine serum (FBS), 1% penicillin-streptomycin (Gibco, Thermo Fisher Scientific, USA), and kept at 37°C in a humidified atmosphere containing 5% CO 2. After reaching 70–80% confluence, the cells were sub-cultured within two-day intervals.
Get Price2020-9-14u2002·u2002Figure 2 . PNS-treated mice exhibited increased energy expenditure and thermogenesis. (A-C) Evaluation of various energy expenditure parameters and results from the average AUC analysis of oxygen consumption (VO 2) (A), carbon dioxide (VCO 2) (B) and heat (expressed as Kcal/h) (C) during a 12-h light/dark cycle in HFD induced obese mice treated with vehicle (HFD, n = 5) or PNS (HFD+PNS, …
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Get Price2016-9-13u2002·u2002KBH-1 is an herbal mixture of Saururus chinensis, Curcuma longa and Polygala tenuifolia. Each herb has been reported to have various pharmaceutical activities; however, the synergistic effect of this herbal composition on obesity has not yet been determined. We investigated the alleviation effect of KBH-1 and its possible molecular mechanism in obesity-induced hepatic steatosis and leptin ...
Get PriceCompositions comprising metabolized conditioned growth medium and/or metabolized cell extract and methods of use are described. The metabolized conditioned growth medium and metabolized cell extrac
Get Price2020-7-1u2002·u2002BG extract in the db/db mice study showed significant difference in the GLUTs, and PPAR expression at liver, and more highly effective result with the extract was observed in muscle. Controlling the level of blood glucose is affected by complex actions by various organs involved the pancreas, fat, muscle, and liver (Saltiel and Kahn, 2001).
Get Price2021-8-23u2002·u2002SCIENTIFIC PROGRAM The program below is subject to change. Monday 23 August 2021 – Day 1 1.45 – 3.30 PM – Plenary sessions 1.30 – 2.15 PM WC11 Virtual Opening Ceremony and Welcome Address The official opening of the WC11 Virtual Congress with speeches from the Chair of the Local Organizing Committee, President of the…
Get Price2013-6-20u2002·u2002Aster glehni (AG) is a Korean traditional herb that grows in Ulleungdo Island, Republic of Korea. None of the several reports on AG include a determination of the effect of AG on adipogenesis. The primary aim of this study was to determine whether AG attenuates adipogenesis in mouse 3T3-L1 cells and epididymal fat tissue. AG blocked the differentiation of 3T3-L1 preadipocytes in a ...
Get Price2.1. The Preparation of Korean Red Ginseng Water Extract. Korean red ginseng water extract was prepared from roots of a 6-year-old ginseng, Panax ginseng C. A. Meyer, harvested in Korea. KRGE was made by steaming fresh ginseng at 90–100°C for 3 h and then drying at 50–80°C. KRGE was prepared from red ginseng water extract, which was extracted at 85~90°C for 8 h of circulating hot water ...
Get Price2021-7-16u2002·u2002NOTE: USDA Export Mark: Exporters may apply the FSIS-issued USDA export mark (e.g. hand stamp or computer-generated sticker) or unique identifier (UI) to: 1) each outside container within a consignment, 2) a securely enclosed pallet or pallets within the consignment (e.g. shrink-wrapped or other effective means), or 3) the closed means of conveyance transporting the consignment (e.g. rail …
Get Price2021-8-26u2002·u2002Propyl gallate - AOX, GRAS, AOX content <0.02% of fat or oil content incl essential oil content of food - 184.1660; Margarine - 166.110; REG, 0.1% alone or/BHA/BHT - Comp of …
Get PriceThe mean droplet size of TENE powder and TENE were 492.2 nm and 323.6 nm, respectively, indicating a single spectrum of size distribution. The curcumin content of TENE powder was 3.3 μg/mg, which was 15 times lower than that of turmeric extract powder (Table 1) (equation 1).
Get Price2021-8-5u2002·u2002Anticancer effect of A. vasica extract against PA 1 cell lines. PA 1 cell lines were grown in Dulbecco's modified Eagle's medium supplemented with fetal bovine serum (FBS), penicillin, and streptomycin. The cells were subcultured after trypsinization with 0.25% in 0.5 mM EDTA and were cultured under 5% CO 2 at 37 °C.
Get Price2018-2-15u2002·u2002Euglena gracilis Z (Euglena) is a unicellular, photosynthesizing, microscopic green alga. It contains several nutrients such as vitamins, minerals, and unsaturated fatty acids. In this study, to verify the potential role of Euglena consumption on human health and obesity, we evaluated the effect of Euglena on human adipose-derived stem cells. We prepared a Euglena extract and evaluated its ...
Get Price2004-11-1u2002·u2002To determine whether shifts in liver fat oxidation potentially contributed to changes in adiposity, we also examined liver CPT activity . Refeeding aP2-transgenic mice the low-calcium diets slightly increased liver CPT activity (44 and 56%), whereas the high-calcium diets increased CPT activity by 150% (P < 0.05).
Get Price2021-1-1u2002·u2002Furthermore, we found a prominent reduction in fat accumulation in Hacd1-KO mice after HFD compared to WT mice. In particular, we observed a blunted increase in white adipose tissue (WAT) weight (Fig. 1G, fig. S1B, and table S1), while a lesser amount of liver steatosis occurred in Hacd1-KO mice as compared with WT mice (fig. S1C).
Get Price2017-10-27u2002·u2002toxic and antigenotoxic dose-dependent,17 antioxidant4,18–20 and antitumoral effects.7,21,22 Mentha cervina L. (Lamiaceae) also known as Hart's penny-royal, is an aromatic herb, found mainly in Eurasia and Africa.
Get Price2018-5-31u2002·u2002Sustarsic et al. reveal that synthesis of the mitochondrial phospholipid cardiolipin is a hallmark of brown and beige fat activation by cold temperature. This single lipid species in thermogenic fat not only shapes adipose mitochondrial bioenergetics but also exerts profound control over whole-body insulin sensitivity and metabolic flexibility.
Get Price2021-7-31u2002·u2002Polyphenolic extracts of green tea, composed primarily of epigallocatechin gallate, protect against EtOH-induced liver damage. Arteel et al. 137 fed rats high-fat diets with and without ethanol ...
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