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The country of origin of the the crude blood (donor cow origin) is the United States. Alternate Names: FBS; FCS; Background Information: The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum (FBS).
Get PriceThe country of origin of the the crude blood (donor cow origin) is the United States. Alternate Names: FBS; FCS; Background Information: The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum (FBS).
Get Price2020-9-15u2002·u2002Background Foetal bovine serum (FBS), is the most commonly used culture medium additive for in vitro cultures, despite its undefined composition, its potential immunogenicity and possible prion/zoonotic transmission. For these reasons, significant efforts have been targeted at finding a substitute, such as serum free-media or human platelet-lysates (hPL). Our aim is to …
Get PriceChlorella vulgaris ( C. vulgaris) is a unicellular microalgae discovered in 1890 by Martinus Willem Beijerinck ( Krienitz et al., 2015 ). It is highly nutritious in nature and thus various forms of C. vulgaris extract are often consumed as a health supplement in some countries, notably Japan.
Get Price2012-2-7u2002·u2002Umbilical cord blood serum (UCBS) is a promising replacement for animal sera for the culture of human mesenchymal stem cells (hMSC), the unique serum composition of UCBS appearing to have variable effects on their proliferation and differentiation. Conditioning UCBS with methods such as charcoal stripping assists specific processes such as adipogenesis and …
Get Price2018-5-11u2002·u2002Fetal bovine serum (FBS) is a well-known growth supplement for cell culture, but xeno-carbohydrates and xeno-proteins contained in FBS may lead to undesired clinical effects [12,13,14,15]. Thus, since 2001, replacement of FBS with suitable alternatives has been recommended for GMP-grade cell expansion throughout Europe and the United States [ 16 ].
Get Price2021-1-1u2002·u2002Fetal bovine serum (FBS; Biowest, Nuaillé, France) was added at a final concentration of 10% to inactivate the enzyme. The cell suspension was filtered through a nylon mesh with a 40-μm pore size (BD Biosciences, San Jose, CA, USA) to remove debris after digestion and centrifuged at 600 × g at 4°C for 7 min.
Get Price2018-4-15u2002·u2002Optimization of chemically defined cell culture media—replacing fetal bovine serum in mammalian in vitro methods Toxicol. In Vitro, 24 ( 2010 ), pp. 1053 - 1063, 10.1016/j.tiv.2010.03.016
Get PriceThe country of origin of the the crude blood (donor cow origin) is the United States. Alternate Names: FBS; FCS; Background Information: The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum …
Get Price2011-7-1u2002·u2002Methods. To identify optimal conditions for in vitro expansion of ECFC, the effects of human endothelial serum-free medium (SFM) supplemented with six pro-angiogenic cytokines and human umbilical cord blood plasma (HCP) were investigated. The in vitro morphology, proliferation, surface antigen expression and in vivo vessel-forming ability were utilized for examining the effects of medium …
Get Price2019-4-22u2002·u2002Human amniotic fluid (hAF) mesenchymal stem cells (MSCs) are commonly cultured in medium containing FBS. However, there are concerns about using animal serum in therapeutic applications due to the potential for immunogenic reactions and the risk of transmission of pathogens. For safety reasons, human platelet lysate (hPL) has been suggested as a replacement for FBS because it …
Get PriceA new cell culture supplement, platelet lysate, was evaluated with reference to fetal bovine serum (FBS), an. established industrial medium for animal cell culture. Chemical and ba cteriological ...
Get Price2007-10-1u2002·u2002During development, cardiac commitment within the mesoderm requires endoderm-secreted factors. Differentiation of embryonic stem cells into the three germ layers in vitro recapitulates developmental processes and can be influenced by supplements added to culture medium. Hence, we investigated the effect of fetal bovine serum (FBS) and KnockOut™ serum replacement (SR) on germ …
Get PriceFetal bovine serum (FBS) is commonly used as a serum supplement for culturing human mesenchymal stromal cells (hMSCs). However, human cells grown in FBS, especially for extended periods, risk potential exposure to bovine immunogenic proteins and infectious agents. To address this issue, we investigated the ability of a novel human platelet serum supplement to substitute for FBS in hMSC …
Get Price2016-7-1u2002·u20027-9 Fetal bovine serum (FBS) can be used for mesenchymal expansion as a source of growth factors in the standard protocol for cellular therapy. 10, 11 Recently, human autologous and allogeneic supplements including platelet derivatives, platelet lysate (PL) and platelet-released factors (PRF) and serum, are assessed in clinical studies to ...
Get Price2018-4-1u2002·u2002The use of fetal bovine serum (FBS) as a cryopreservation supplement is not suitable for the banking of mesenchymal stem cells (MSCs) due to the risk of transmission of disease as well as xenogeneic immune reactions in the transplanted host. ... the efficiency of human serum (HS) and knockout serum replacement (KSR) for cryopreservation of ASCs ...
Get Price2013-9-17u2002·u2002In this study, in order to make the best of umbilical cord blood, the human UCBP was dialyzed to replace fetal bovine serum (FBS) in the culture medium. hUC-MSCs were cultured in the new medium. Cell growth rate, specific biomarkers, and differentiation properties were detected to characterize the cell proliferation and MSC-specific properties.
Get PriceTwo types of serum were used in the present study, human umbilical cord blood-derived serum (hUCS) and fetal bovine serum (FBS). The hUCS was collected by our collaborator and the serum collecting protocol was described previously [16, 17 16, 17]. HyClone FBS (recommended by the provider of human foreskin fibroblasts) was purchased from Thermo ...
Get PriceThe country of origin of the the crude blood (donor cow origin) is the United States. Alternate Names: FBS; FCS; Background Information: The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum …
Get Price2011-7-1u2002·u2002Methods. To identify optimal conditions for in vitro expansion of ECFC, the effects of human endothelial serum-free medium (SFM) supplemented with six pro-angiogenic cytokines and human umbilical cord blood plasma (HCP) were investigated. The in vitro morphology, proliferation, surface antigen expression and in vivo vessel-forming ability were utilized for examining the effects of medium …
Get Price2019-4-22u2002·u2002Human amniotic fluid (hAF) mesenchymal stem cells (MSCs) are commonly cultured in medium containing FBS. However, there are concerns about using animal serum in therapeutic applications due to the potential for immunogenic reactions and the risk of transmission of pathogens. For safety reasons, human platelet lysate (hPL) has been suggested as a replacement for FBS because it …
Get PriceA new cell culture supplement, platelet lysate, was evaluated with reference to fetal bovine serum (FBS), an. established industrial medium for animal cell culture. Chemical and ba cteriological ...
Get Price2007-10-1u2002·u2002During development, cardiac commitment within the mesoderm requires endoderm-secreted factors. Differentiation of embryonic stem cells into the three germ layers in vitro recapitulates developmental processes and can be influenced by supplements added to culture medium. Hence, we investigated the effect of fetal bovine serum (FBS) and KnockOut™ serum replacement (SR) on germ …
Get PriceFetal bovine serum (FBS) is commonly used as a serum supplement for culturing human mesenchymal stromal cells (hMSCs). However, human cells grown in FBS, especially for extended periods, risk potential exposure to bovine immunogenic proteins and infectious agents. To address this issue, we investigated the ability of a novel human platelet serum supplement to substitute for FBS in hMSC …
Get Price2016-7-1u2002·u20027-9 Fetal bovine serum (FBS) can be used for mesenchymal expansion as a source of growth factors in the standard protocol for cellular therapy. 10, 11 Recently, human autologous and allogeneic supplements including platelet derivatives, platelet lysate (PL) and platelet-released factors (PRF) and serum, are assessed in clinical studies to ...
Get Price2018-4-1u2002·u2002The use of fetal bovine serum (FBS) as a cryopreservation supplement is not suitable for the banking of mesenchymal stem cells (MSCs) due to the risk of transmission of disease as well as xenogeneic immune reactions in the transplanted host. ... the efficiency of human serum (HS) and knockout serum replacement (KSR) for cryopreservation of ASCs ...
Get Price2013-9-17u2002·u2002In this study, in order to make the best of umbilical cord blood, the human UCBP was dialyzed to replace fetal bovine serum (FBS) in the culture medium. hUC-MSCs were cultured in the new medium. Cell growth rate, specific biomarkers, and differentiation properties were detected to characterize the cell proliferation and MSC-specific properties.
Get PriceTwo types of serum were used in the present study, human umbilical cord blood-derived serum (hUCS) and fetal bovine serum (FBS). The hUCS was collected by our collaborator and the serum collecting protocol was described previously [16, 17 16, 17]. HyClone FBS (recommended by the provider of human foreskin fibroblasts) was purchased from Thermo ...
Get PriceThe country of origin of the the crude blood (donor cow origin) is the United States. Alternate Names: FBS; FCS; Background Information: The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum …
Get Price2011-7-1u2002·u2002Methods. To identify optimal conditions for in vitro expansion of ECFC, the effects of human endothelial serum-free medium (SFM) supplemented with six pro-angiogenic cytokines and human umbilical cord blood plasma (HCP) were investigated. The in vitro morphology, proliferation, surface antigen expression and in vivo vessel-forming ability were utilized for examining the effects of medium …
Get Price2019-4-22u2002·u2002Human amniotic fluid (hAF) mesenchymal stem cells (MSCs) are commonly cultured in medium containing FBS. However, there are concerns about using animal serum in therapeutic applications due to the potential for immunogenic reactions and the risk of transmission of pathogens. For safety reasons, human platelet lysate (hPL) has been suggested as a replacement for FBS because it …
Get PriceA new cell culture supplement, platelet lysate, was evaluated with reference to fetal bovine serum (FBS), an. established industrial medium for animal cell culture. Chemical and ba cteriological ...
Get Price2007-10-1u2002·u2002During development, cardiac commitment within the mesoderm requires endoderm-secreted factors. Differentiation of embryonic stem cells into the three germ layers in vitro recapitulates developmental processes and can be influenced by supplements added to culture medium. Hence, we investigated the effect of fetal bovine serum (FBS) and KnockOut™ serum replacement (SR) on germ …
Get PriceFetal bovine serum (FBS) is commonly used as a serum supplement for culturing human mesenchymal stromal cells (hMSCs). However, human cells grown in FBS, especially for extended periods, risk potential exposure to bovine immunogenic proteins and infectious agents. To address this issue, we investigated the ability of a novel human platelet serum supplement to substitute for FBS in hMSC …
Get Price2016-7-1u2002·u20027-9 Fetal bovine serum (FBS) can be used for mesenchymal expansion as a source of growth factors in the standard protocol for cellular therapy. 10, 11 Recently, human autologous and allogeneic supplements including platelet derivatives, platelet lysate (PL) and platelet-released factors (PRF) and serum, are assessed in clinical studies to ...
Get Price2018-4-1u2002·u2002The use of fetal bovine serum (FBS) as a cryopreservation supplement is not suitable for the banking of mesenchymal stem cells (MSCs) due to the risk of transmission of disease as well as xenogeneic immune reactions in the transplanted host. ... the efficiency of human serum (HS) and knockout serum replacement (KSR) for cryopreservation of ASCs ...
Get Price2013-9-17u2002·u2002In this study, in order to make the best of umbilical cord blood, the human UCBP was dialyzed to replace fetal bovine serum (FBS) in the culture medium. hUC-MSCs were cultured in the new medium. Cell growth rate, specific biomarkers, and differentiation properties were detected to characterize the cell proliferation and MSC-specific properties.
Get PriceTwo types of serum were used in the present study, human umbilical cord blood-derived serum (hUCS) and fetal bovine serum (FBS). The hUCS was collected by our collaborator and the serum collecting protocol was described previously [16, 17 16, 17]. HyClone FBS (recommended by the provider of human foreskin fibroblasts) was purchased from Thermo ...
Get Price2020-9-15u2002·u2002Foetal bovine serum (FBS), is the most commonly used culture medium additive for in vitro cultures, despite its undefined composition, its potential immunogenicity and possible prion/zoonotic transmission. For these reasons, significant efforts have been targeted at finding a substitute, such as serum free-media or human platelet-lysates (hPL).
Get Price2015-1-15u2002·u2002Cell therapy using human mesenchymal stem cells (MSCs) is an attractive approach for many refractory diseases. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are considered as a favorable tool due to its abundance in the body, easy proliferation, and high cytokine production potency. In order to avoid the risks associated with the use of fetal bovine serum …
Get Price2015-12-3u2002·u2002Medium 'A' was our previously published protocol and consisted of alpha-MEM enriched with 20% of fetal bovine serum and recombinant human cytokines SCF 5ng/mL, Flt3L 5ng/mL, IL-7 5ng/mL, and IL-15 10ng/mL. Medium 'B' was AIM V enriched with 10% of human AB serum and cytokines SCF 5ng/mL, Flt3L 5ng/mL, IL-7 20ng/mL, and IL-15 50ng/mL.
Get Price2019-8-29u2002·u2002Cells cultured with CBS had a significantly higher expansion rate than fetal bovine serum (FBS), whereas there was no significant difference observed among different CBS blood groups. *p < 0.05, respectively compared with each CBS group. (B) Comparison of the fold expansion rates for hPDMSCs isolated from different blood group donors.
Get Price2019-3-7u2002·u2002Mesenchymal stromal cells (MSCs) are considered potential candidates that hold great promise in the treatment of immune-related diseases. For therapeutic applications, it is necessary to isolate and expand MSCs with procedures complying with good manufacturing practice (GMP). Recent studies reported the use of human serum (HS) instead of fetal bovine serum (FBS) for the …
Get PriceSerum is the liquid form blood component prepared by centrifuging the whole collected blood from animals. In culture medium serum used to enhance the nutritional content of the medium for cell ...
Get Price2021-2-11u2002·u2002High concentrations of serum have been reported to inhibit differentiation . X-VIVO™ 15 is a serum-free medium, which has been reported to improve cell differentiation . Hence, the effects of serum (RPMI-1640 supplemented with 10% FBS) and serum-free medium (X-VIVO™ 15 medium) on the differentiation of HL-60 cells were also compared.
Get Price2013-10-30u2002·u2002Multilineage differentiation, immunomodulation and secretion of trophic factors render mesenchymal stromal cells (MSC) highly attractive for clinical application. Human platelet derivatives such as pooled human platelet lysate (pHPL) and thrombin-activated platelet releasate in plasma (tPRP) have been introduced as alternatives to fetal bovine serum (FBS) to achieve …
Get PriceAbstract. Although the biological properties of mesenchymal stem cells (MSC) are well-characterized in vitro, MSC clinical application is still far away to be achieved, mainly due to the need of xenogeneic substances for cell expansion, such as fetal bovine serum (FBS).FBS presents risks regarding pathogens transmissions and internalization of animal's proteins, which can …
Get PriceSmartphone based on-chip fluorescence imaging and capillary flow velocity measurement for detecting ROR1+ cancer cells from buffy coat blood samples on dual-layer paper microfluidic chip Tiffany-Heather Ulep, Ryan Zenhausern, Alana Gonzales, David S.Knoff, Paula Lengerke Diaz, Januario E. Castro, Jeong-Yeol Yoon
Get PriceThe country of origin of the the crude blood (donor cow origin) is the United States. Alternate Names: FBS; FCS; Background Information: The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum …
Get Price2011-7-1u2002·u2002Methods. To identify optimal conditions for in vitro expansion of ECFC, the effects of human endothelial serum-free medium (SFM) supplemented with six pro-angiogenic cytokines and human umbilical cord blood plasma (HCP) were investigated. The in vitro morphology, proliferation, surface antigen expression and in vivo vessel-forming ability were utilized for examining the effects of medium …
Get Price2019-4-22u2002·u2002Human amniotic fluid (hAF) mesenchymal stem cells (MSCs) are commonly cultured in medium containing FBS. However, there are concerns about using animal serum in therapeutic applications due to the potential for immunogenic reactions and the risk of transmission of pathogens. For safety reasons, human platelet lysate (hPL) has been suggested as a replacement for FBS because it …
Get PriceA new cell culture supplement, platelet lysate, was evaluated with reference to fetal bovine serum (FBS), an. established industrial medium for animal cell culture. Chemical and ba cteriological ...
Get Price2007-10-1u2002·u2002During development, cardiac commitment within the mesoderm requires endoderm-secreted factors. Differentiation of embryonic stem cells into the three germ layers in vitro recapitulates developmental processes and can be influenced by supplements added to culture medium. Hence, we investigated the effect of fetal bovine serum (FBS) and KnockOut™ serum replacement (SR) on germ …
Get PriceFetal bovine serum (FBS) is commonly used as a serum supplement for culturing human mesenchymal stromal cells (hMSCs). However, human cells grown in FBS, especially for extended periods, risk potential exposure to bovine immunogenic proteins and infectious agents. To address this issue, we investigated the ability of a novel human platelet serum supplement to substitute for FBS in hMSC …
Get Price2016-7-1u2002·u20027-9 Fetal bovine serum (FBS) can be used for mesenchymal expansion as a source of growth factors in the standard protocol for cellular therapy. 10, 11 Recently, human autologous and allogeneic supplements including platelet derivatives, platelet lysate (PL) and platelet-released factors (PRF) and serum, are assessed in clinical studies to ...
Get Price2018-4-1u2002·u2002The use of fetal bovine serum (FBS) as a cryopreservation supplement is not suitable for the banking of mesenchymal stem cells (MSCs) due to the risk of transmission of disease as well as xenogeneic immune reactions in the transplanted host. ... the efficiency of human serum (HS) and knockout serum replacement (KSR) for cryopreservation of ASCs ...
Get Price2013-9-17u2002·u2002In this study, in order to make the best of umbilical cord blood, the human UCBP was dialyzed to replace fetal bovine serum (FBS) in the culture medium. hUC-MSCs were cultured in the new medium. Cell growth rate, specific biomarkers, and differentiation properties were detected to characterize the cell proliferation and MSC-specific properties.
Get PriceTwo types of serum were used in the present study, human umbilical cord blood-derived serum (hUCS) and fetal bovine serum (FBS). The hUCS was collected by our collaborator and the serum collecting protocol was described previously [16, 17 16, 17]. HyClone FBS (recommended by the provider of human foreskin fibroblasts) was purchased from Thermo ...
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