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  • Stem Cells - sigmaaldrich.com

    Serum-free Stem Cell Media and Supplements. Historically, fetal bovine serum has been the supplement of choice for in vitro mammalian cell culture including stem cells. However, due to the undefined composition of FBS, researchers have transitioned to more chemically defined serum-free and xeno-free media and supplements for stem cell research ...

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  • philippines healthy newborn calf serum replacement business

    Fetal Calf Serum . Thermo Fisher fetal calf serum Gibco Calf Serum with Iron US Origin is collected from calves 1 6 months of age which naturally have higher levels of transferrin to bind iron The superior levels of transferrin promote higher performance in cell culture applications Key features • Iron content 500 700 µg dL • Triple 0 1 micron sterile filtered • Cost effective • US ...

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  • Cytotoxicity of Various Endodontic Materials on Stem Cells ...

    2015-12-24u2002·u2002To obtain higher number of cells, they were cultured again in a culture medium containing 15% fetal bovine serum (FBS) (Gibco BRI, Grand Island, NY, USA). This cell line was cultured in sterile culture medium containing 10% bovine serum (DMEM) in sterile cell culture flasks (SPL Life Science, Gyeonggi-do, South Korea).

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  • Cell Freezing Reagents - sigmaaldrich.com

    Protect valuable cell line stocks by choosing reagents that are designed to protect cells during ultralow temperature storage. Our DMSO selection is application-tested, and ready-to-use cell freezing media are available in DMSO-free and in variable DMSO concentrations.

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  • 779 questions with answers in FETAL BOVINE SERUM

    2021-9-1u2002·u2002Review and cite FETAL BOVINE SERUM protocol, troubleshooting and other methodology information Contact experts in FETAL BOVINE SERUM to get answers

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  • Alzheimer's Disease Drug Discovery from Herbs ...

    2011-9-13u2002·u2002using a sterile pipette. The resulting cell suspension was cen-trifuged at 2000 rpm for 5 minutes. The neurons were seeded into poly-L-lysine-coated 96-well tissue culture plates at a final density of 1.5 105 cells/cm2. The cultures were main-tained in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum, 10% horse serum ...

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  • Membrane-reinforced three-dimensional electrospun silk ...

    water to replace methanol with water. 2.3. Fabrication of membrane-reinforced 3D electrospun SF scaffolds Aqueous SF solutions were prepared at different concentrations from 0 to 5%, (SF/water, w/v). HAP particles (Bio Alpha Inc., Sungnam, South Korea) were added to …

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  • Biodegradable nanofiber bone-tissue scaffold as remotely ...

    2020-10-1u2002·u2002The PLLA films were 1 cm long by 1 cm wide. Each piece was placed in a separate well of a 24-well plate and the well was filled with 0.5 ml of standard cell media (DMEM, 10% fetal bovine serum and 1% antibiotic solution pennstep containing 50% penicillin and 50% streptomycin, all three components purchased from Gibco).

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  • Aphrodisiac Property of the Aqueous Extract of Cynanchum ...

    2020-8-21u2002·u2002The purpose of the current study was to investigate the total phenolic content and the antioxidant activity of the aqueous extract of Cynanchum wilfordii Radix (CWW). In addition, we conducted in vitro and in vivo tests to examine whether the aqueous extract of CWW has an aphrodisiac property. The results indicated significant increases in testosterone synthesis in …

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  • Glass Bottles

    VWR® Square Laboratory Bottles, Wide Neck, with GL80 Thread. Supplier: VWR International. Description: Made from borosilicate 33 expansion glass, these bottles are available in both Clear and Amber color formats. Certificates.,75871-424CS,75871-420CS,75871-304CS,75871-310CS,75871-426CS,75871-422CS,75871-306CS.

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  • Cytotoxicity of Various Endodontic Materials on Stem Cells ...

    2015-12-24u2002·u2002To obtain higher number of cells, they were cultured again in a culture medium containing 15% fetal bovine serum (FBS) (Gibco BRI, Grand Island, NY, USA). This cell line was cultured in sterile culture medium containing 10% bovine serum (DMEM) in sterile cell culture flasks (SPL Life Science, Gyeonggi-do, South Korea).

    Get Price
  • Defined serum‐free three‐dimensional culture of umbilical ...

    2020-12-25u2002·u2002fetal bovine serum GCCP good cell culture practice GMP good manufacturing practice HGF hepatocyte growth factor HDF human dermal fibroblasts HLA‐DR human leukocyte antigen—DR isotype hPL human platelet lysate HS human serum KO KnockOut serum replacement MTT 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide NTA ...

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  • Bio-inspired multifunctional collagen/electrospun ...

    2021-7-1u2002·u2002To examine the osteoblast markers mRNAs expression, equally weighed membranes were placed in 6 well plates and treated with DMEM containing 10% fetal bovine serum. Subsequently, 1 mL of MG63 cell solution (3 × 10 5 cells/mL) was added to the membrane and incubated at standard culture conditions. The samples were cryo pulverized and total RNA ...

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  • Frontiers

    Salmonella enterica subsp. enterica serovar Gallinarum (SG) is a common pathogen in chickens, and causes an acute systemic disease that leads to high mortality. The live attenuated vaccine 9R is able to successfully protect chickens older than six weeks by activating a robust cell-mediated immune response, but its safety and efficacy in young chickens remains controversial. An inactivated SG ...

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  • An Alternative Culture Method to Maintain Genomic ...

    Two groups seminally established the mouse ES cell lines in 1981 and when cells derived from the early mouse embryo were cultured in fetal bovine serum (FBS)-containing medium with mouse embryonic fibroblasts (MEFs) as the feeder layer 1, 4. MEFs were inactivated mitotically and were pre-grown in dishes prior to culturing ES cells.

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  • Aphrodisiac Property of the Aqueous Extract of

    2020-8-21u2002·u2002The purpose of the current study was to investigate the total phenolic content and the antioxidant activity of the aqueous extract of Cynanchum wilfordii Radix (CWW). In addition, we conducted in vitro and in vivo tests to examine whether the aqueous extract of CWW has an aphrodisiac property. The results indicated significant increases in testosterone synthesis in Leydig TM3 cells …

    Get Price
  • Alzheimer's Disease Drug Discovery from Herbs ...

    2011-9-13u2002·u2002using a sterile pipette. The resulting cell suspension was cen-trifuged at 2000 rpm for 5 minutes. The neurons were seeded into poly-L-lysine-coated 96-well tissue culture plates at a final density of 1.5 105 cells/cm2. The cultures were main-tained in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum, 10% horse serum ...

    Get Price
  • Biocompatibility effects of biologically synthesized ...

    2013-9-23u2002·u2002After a 24-h exposure, the cells were washed twice with serum-free DMEM, and 15 μL of WST-8 solution was added to each well containing 100 μL of serum-free DMEM. After 1 h of incubation at 37°C under 5% CO 2, 80 μL of the mixture was transferred to another 96-well plate because residual GO or S-rGO can affect the absorbance values at 450 nm.

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  • Shape-Depended Biological Properties of Ag3PO4 ...

    2019-11-20u2002·u2002A solution of aqueous ammonia was added to the solution thus prepared to obtain a brown solid completely dissolved in the solution. The next step was to add 0.1639 g of Na 3 PO 4 dissolved in 30 mL of deionized water. After stirring for 5 minutes, the precipitate was collected, washed several times with deionized water, and dried in a desiccator.

    Get Price
  • Bosentan - ToxBank Gold Compounds and Biological

    2017-8-18u2002·u2002Fetal Bovine Serum (10500-064) Fetal Calf Serum (P103009) Fibronectin, from bovine plasma (F1141-5MG) Fungizone® Antimycotic, Liquid (15290-018) GenElute™ Mammalian Total RNA Miniprep Kit (RTN350) GenElute™ PCR Clean-Up Kit (NA1020) Gentamycin (P06-13001) Glycine sodium salt hydrate (G6761) Ham's F-12 Nutrient Mix (21765)

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  • Cytotoxicity of Various Endodontic Materials on Stem Cells ...

    2015-12-24u2002·u2002To obtain higher number of cells, they were cultured again in a culture medium containing 15% fetal bovine serum (FBS) (Gibco BRI, Grand Island, NY, USA). This cell line was cultured in sterile culture medium containing 10% bovine serum (DMEM) in sterile cell culture flasks (SPL Life Science, Gyeonggi-do, South Korea).

    Get Price
  • Effects of the Aqueous Extract of Epimedii Herba on the ...

    2002-7-25u2002·u2002and blocked with 1% bovine serum albumin (BSA)/PBS at room temperature for 2h. This buffer solution was also used as a diluent in all subsequent steps of the ELISAs. After three washings of the blocked wells with PBS-Tween, 100ml of appropriately diluted serum sample was added to duplicate wells. As a standard serum we used 10 serial two-fold dilu-

    Get Price
  • Bo-Gan-Whan regulates proliferation and migration of ...

    2017-4-10u2002·u2002University Oriental Hospital, Korea which has been pre-pared specifically for this study from the commercially-available products. The plants were blended (a total of 30 g), and the crude powder was boiled in 1000 mL of sterile deionized water at 100 °C for 3 h. The aqueous ex-tracts were concentrated and evaporated at 60 °C under vacuum.

    Get Price
  • Aphrodisiac Property of the Aqueous Extract of

    2020-8-21u2002·u2002The purpose of the current study was to investigate the total phenolic content and the antioxidant activity of the aqueous extract of Cynanchum wilfordii Radix (CWW). In addition, we conducted in vitro and in vivo tests to examine whether the aqueous extract of CWW has an aphrodisiac property. The results indicated significant increases in testosterone synthesis in Leydig TM3 cells …

    Get Price
  • Biocompatibility effects of biologically synthesized ...

    2013-9-23u2002·u2002After a 24-h exposure, the cells were washed twice with serum-free DMEM, and 15 μL of WST-8 solution was added to each well containing 100 μL of serum-free DMEM. After 1 h of incubation at 37°C under 5% CO 2, 80 μL of the mixture was transferred to another 96-well plate because residual GO or S-rGO can affect the absorbance values at 450 nm.

    Get Price
  • 2020 PRODUCT CATALOG - ALK

    2020-1-2u2002·u20026 Call Sales Support at 800-325-7354 PRE-PEN® (benzylpenicilloyl polylysine injection USP) is manufactured by AllerQuest, LLC, and distributed exclusively by ALK. PRE-PEN® (benzylpenicilloyl polylysine injection USP) is a clear, colorless, sterile solution supplied in 0.25 mL sealed glass ampules. Each ampule is sufficient to evaluate one patient by skin prick (percutaneous) test and ...

    Get Price
  • Shape-Depended Biological Properties of Ag3PO4 ...

    2019-11-20u2002·u2002A solution of aqueous ammonia was added to the solution thus prepared to obtain a brown solid completely dissolved in the solution. The next step was to add 0.1639 g of Na 3 PO 4 dissolved in 30 mL of deionized water. After stirring for 5 minutes, the precipitate was collected, washed several times with deionized water, and dried in a desiccator.

    Get Price
  • Use of graphene as protection film in biological ...

    2014-2-14u2002·u2002Due to their strength and excellent resistance to fatigue degradation, metals are extensively used in medical fields such as bone and joint replacements 1,2,3, stents 4,5, dental materials 6,7 and ...

    Get Price
  • EMULSIFICATION TECHNOLOGY - Microspheres for

    The shear forces used in membrane emulsification are less than 50% of those required to lyse viable cells. This gentle process results in almost no degradation of sensitive APIs, retaining well above 90% biological activity compared with 37% to 67% degradation when rotor-stator hoxadmogenization is the method chosen for mixadcrosphere encapsulation. 7 Because Micropore's process is an ...

    Get Price
  • BCL-2 Protein family and the regulation of programmed

    2021-9-22u2002·u2002The Sp-family of transcription factors regulate variety of genes involved in critical processes ranging from cell cycle, proliferation, cell differentiation, apoptosis and associated with a number of human cancers [22C26]. Sp1 is definitely a negative prognostic element for survival in some cancer individuals [27, 28].

    Get Price
  • :: JVS :: Journal of Veterinary Science

    Briefly, an H 3 PO 4 aqueous solution (5 dm 3, 0.67 M) was gradually added to a 1 M Ca(OH) 2 suspension with vigorous stirring. pH of the reaction was maintained at 6~7 by the addition of a 28% ammonium aqueous solution. The precipitate was dried at 120℃ and burned at 800℃.

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  • Lonza, Enabling a Healthier World

    About Lonza. At Lonza, we enable a healthier world by supporting our healthcare customers on the path to commercialization. Our community of 15,000 skilled employees work across a global network of more than 30 sites to deliver for our customers across the pharma, biotech and nutrition markets. By combining technological insight with world ...

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  • Choline chloride

    Choline chloride appears as white crystals. Practically neutral aqueous solution. (NTP, 1992) CAMEO Chemicals. Choline chloride is a quaternary ammonium salt with choline cation and chloride anion. It has a role as an animal growth promotant. It is a chloride salt and a quaternary ammonium salt. It contains a choline.

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  • Alzheimer's Disease Drug Discovery from Herbs ...

    2011-9-13u2002·u2002using a sterile pipette. The resulting cell suspension was cen-trifuged at 2000 rpm for 5 minutes. The neurons were seeded into poly-L-lysine-coated 96-well tissue culture plates at a final density of 1.5 105 cells/cm2. The cultures were main-tained in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum, 10% horse serum ...

    Get Price
  • Sequentially Programmable and Cellularly Selective ...

    Cholinesterase is also distributed in serum 47 and 10% fetal bovine serum (FBS) is used in cell culturing process. It is therefore necessary to examine the influence of cholinesterase in cell medium on the programmable assembly process. 95% DC was incubated in cell medium at 37 °C for 12 h, and followed by the same operating procedure.

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  • Sensory neurons and osteoblasts: close partners in a ...

    2014-3-28u2002·u2002To prepare in situ crosslinking hydrogel matrices, an internal gelation strategy adapted from Kuo et al. was used. 49 The alginate solution was sterile-filtered (0.22 μm) and thoroughly mixed with an aqueous suspension of CaCO 3 (Fluka) at a CaCO 3 /COOH molar ratio of 1.6. To trigger gel formation, a fresh solution of glucone delta-lactone ...

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  • Heracleum moellendorffii root extracts exert ...

    2020-11-16u2002·u2002Heracleum moellendorffii (H. moellendorffii) is a family of Umbelliferae and has long been used for food and medicinal purposes.However, the immune-enhancing activity of H. moellendorffii has not been studied. Thus, we evaluated in vitro immune-enhancing activity of H. moellendorffii through macrophage activation using RAW264.7 cells.Heracleum moellendorffii …

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  • Marine Drugs

    The objective of this work was to develop sustained-release Ca-alginate beads of apigenin using sodium alginate, a natural polysaccharide. Six batches were prepared by applying the ionotropic gelation technique, wherein calcium chloride was used as a crosslinking agent. The beads were evaluated for particle size, drug loading, percentage yield, and in vitro drug release.

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  • Shape-Depended Biological Properties of Ag3PO4 ...

    2019-11-20u2002·u2002A solution of aqueous ammonia was added to the solution thus prepared to obtain a brown solid completely dissolved in the solution. The next step was to add 0.1639 g of Na 3 PO 4 dissolved in 30 mL of deionized water. After stirring for 5 minutes, the precipitate was collected, washed several times with deionized water, and dried in a desiccator.

    Get Price
  • Pharmceutical composition for treatment of wounds ...

    For the fetal bovine serum powder, the test group showed 152.62 μm±20.86 which was significantly different from the control group of 41.20 μm±7.44 (p<0.01). For the fetal bovine serum-containing ointment, the test group showed 168.62 μm±19.26 which was significantly different from the control group of 58.62 μm±7.62 (p<0.01).

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  • Cytotoxicity of Various Endodontic Materials on Stem Cells ...

    2015-12-24u2002·u2002To obtain higher number of cells, they were cultured again in a culture medium containing 15% fetal bovine serum (FBS) (Gibco BRI, Grand Island, NY, USA). This cell line was cultured in sterile culture medium containing 10% bovine serum (DMEM) in sterile cell culture flasks (SPL Life Science, Gyeonggi-do, South Korea).

    Get Price
  • Effects of the Aqueous Extract of Epimedii Herba on the ...

    2002-7-25u2002·u2002and blocked with 1% bovine serum albumin (BSA)/PBS at room temperature for 2h. This buffer solution was also used as a diluent in all subsequent steps of the ELISAs. After three washings of the blocked wells with PBS-Tween, 100ml of appropriately diluted serum sample was added to duplicate wells. As a standard serum we used 10 serial two-fold dilu-

    Get Price
  • Bo-Gan-Whan regulates proliferation and migration of ...

    2017-4-10u2002·u2002University Oriental Hospital, Korea which has been pre-pared specifically for this study from the commercially-available products. The plants were blended (a total of 30 g), and the crude powder was boiled in 1000 mL of sterile deionized water at 100 °C for 3 h. The aqueous ex-tracts were concentrated and evaporated at 60 °C under vacuum.

    Get Price
  • Aphrodisiac Property of the Aqueous Extract of

    2020-8-21u2002·u2002The purpose of the current study was to investigate the total phenolic content and the antioxidant activity of the aqueous extract of Cynanchum wilfordii Radix (CWW). In addition, we conducted in vitro and in vivo tests to examine whether the aqueous extract of CWW has an aphrodisiac property. The results indicated significant increases in testosterone synthesis in …

    Get Price
  • Biocompatibility effects of biologically synthesized ...

    2013-9-23u2002·u2002After a 24-h exposure, the cells were washed twice with serum-free DMEM, and 15 μL of WST-8 solution was added to each well containing 100 μL of serum-free DMEM. After 1 h of incubation at 37°C under 5% CO 2, 80 μL of the mixture was transferred to another 96-well plate because residual GO or S-rGO can affect the absorbance values at 450 nm.

    Get Price
  • 2020 PRODUCT CATALOG - ALK

    2020-1-2u2002·u20026 Call Sales Support at 800-325-7354 PRE-PEN® (benzylpenicilloyl polylysine injection USP) is manufactured by AllerQuest, LLC, and distributed exclusively by ALK. PRE-PEN® (benzylpenicilloyl polylysine injection USP) is a clear, colorless, sterile solution supplied in 0.25 mL sealed glass ampules. Each ampule is sufficient to evaluate one patient by skin prick …

    Get Price
  • Shape-Depended Biological Properties of Ag3PO4 ...

    2019-11-20u2002·u2002A solution of aqueous ammonia was added to the solution thus prepared to obtain a brown solid completely dissolved in the solution. The next step was to add 0.1639 g of Na 3 PO 4 dissolved in 30 mL of deionized water. After stirring for 5 minutes, the precipitate was collected, washed several times with deionized water, and dried in a desiccator.

    Get Price
  • Use of graphene as protection film in biological ...

    2014-2-14u2002·u2002Due to their strength and excellent resistance to fatigue degradation, metals are extensively used in medical fields such as bone and joint replacements 1,2,3, stents 4,5, dental materials 6,7 and ...

    Get Price
  • EMULSIFICATION TECHNOLOGY - Microspheres for

    The shear forces used in membrane emulsification are less than 50% of those required to lyse viable cells. This gentle process results in almost no degradation of sensitive APIs, retaining well above 90% biological activity compared with 37% to 67% degradation when rotor-stator hoxadmogenization is the method chosen for mixadcrosphere encapsulation. 7 Because Micropore's …

    Get Price
  • BCL-2 Protein family and the regulation of programmed

    2021-9-22u2002·u2002The Sp-family of transcription factors regulate variety of genes involved in critical processes ranging from cell cycle, proliferation, cell differentiation, apoptosis and associated with a number of human cancers [22C26]. Sp1 is definitely a negative prognostic element for survival in some cancer individuals [27, 28].

    Get Price
  • Cytotoxicity of Various Endodontic Materials on Stem Cells ...

    2015-12-24u2002·u2002To obtain higher number of cells, they were cultured again in a culture medium containing 15% fetal bovine serum (FBS) (Gibco BRI, Grand Island, NY, USA). This cell line was cultured in sterile culture medium containing 10% bovine serum (DMEM) in sterile cell culture flasks (SPL Life Science, Gyeonggi-do, South Korea).

    Get Price
  • Effects of the Aqueous Extract of Epimedii Herba on the ...

    2002-7-25u2002·u2002and blocked with 1% bovine serum albumin (BSA)/PBS at room temperature for 2h. This buffer solution was also used as a diluent in all subsequent steps of the ELISAs. After three washings of the blocked wells with PBS-Tween, 100ml of appropriately diluted serum sample was added to duplicate wells. As a standard serum we used 10 serial two-fold dilu-

    Get Price
  • Bo-Gan-Whan regulates proliferation and migration of ...

    2017-4-10u2002·u2002University Oriental Hospital, Korea which has been pre-pared specifically for this study from the commercially-available products. The plants were blended (a total of 30 g), and the crude powder was boiled in 1000 mL of sterile deionized water at 100 °C for 3 h. The aqueous ex-tracts were concentrated and evaporated at 60 °C under vacuum.

    Get Price
  • Aphrodisiac Property of the Aqueous Extract of

    2020-8-21u2002·u2002The purpose of the current study was to investigate the total phenolic content and the antioxidant activity of the aqueous extract of Cynanchum wilfordii Radix (CWW). In addition, we conducted in vitro and in vivo tests to examine whether the aqueous extract of CWW has an aphrodisiac property. The results indicated significant increases in testosterone synthesis in …

    Get Price
  • Biocompatibility effects of biologically synthesized ...

    2013-9-23u2002·u2002After a 24-h exposure, the cells were washed twice with serum-free DMEM, and 15 μL of WST-8 solution was added to each well containing 100 μL of serum-free DMEM. After 1 h of incubation at 37°C under 5% CO 2, 80 μL of the mixture was transferred to another 96-well plate because residual GO or S-rGO can affect the absorbance values at 450 nm.

    Get Price
  • 2020 PRODUCT CATALOG - ALK

    2020-1-2u2002·u20026 Call Sales Support at 800-325-7354 PRE-PEN® (benzylpenicilloyl polylysine injection USP) is manufactured by AllerQuest, LLC, and distributed exclusively by ALK. PRE-PEN® (benzylpenicilloyl polylysine injection USP) is a clear, colorless, sterile solution supplied in 0.25 mL sealed glass ampules. Each ampule is sufficient to evaluate one patient by skin prick …

    Get Price
  • Shape-Depended Biological Properties of Ag3PO4 ...

    2019-11-20u2002·u2002A solution of aqueous ammonia was added to the solution thus prepared to obtain a brown solid completely dissolved in the solution. The next step was to add 0.1639 g of Na 3 PO 4 dissolved in 30 mL of deionized water. After stirring for 5 minutes, the precipitate was collected, washed several times with deionized water, and dried in a desiccator.

    Get Price
  • Use of graphene as protection film in biological ...

    2014-2-14u2002·u2002Due to their strength and excellent resistance to fatigue degradation, metals are extensively used in medical fields such as bone and joint replacements 1,2,3, stents 4,5, dental materials 6,7 and ...

    Get Price
  • EMULSIFICATION TECHNOLOGY - Microspheres for

    The shear forces used in membrane emulsification are less than 50% of those required to lyse viable cells. This gentle process results in almost no degradation of sensitive APIs, retaining well above 90% biological activity compared with 37% to 67% degradation when rotor-stator hoxadmogenization is the method chosen for mixadcrosphere encapsulation. 7 Because Micropore's …

    Get Price
  • BCL-2 Protein family and the regulation of programmed

    2021-9-22u2002·u2002The Sp-family of transcription factors regulate variety of genes involved in critical processes ranging from cell cycle, proliferation, cell differentiation, apoptosis and associated with a number of human cancers [22C26]. Sp1 is definitely a negative prognostic element for survival in some cancer individuals [27, 28].

    Get Price